Renin activity

Renin is produced, stored and secreted by the cells adjacent to the glomerulus. It is a hydrolyzed protease that can convert angiotensinogen to angiotensin I and form angiotensin II by the action of invertase. The renin-angiotensin system plays an important role in the regulation of blood pressure, water and electrolyte balance in the body. Basic Information Specialist classification: growth and development examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Tips: Determination of plasma renin activity, the diet of the subject should control sodium and potassium intake, the standard diet is daily: sodium 100mmol, potassium 60 ~ 100mmol. Normal value 1.0 to 2.5 μg·L-1/h. Clinical significance Reduced in primary hypertension, low renin, primary aldosteronism, pseudohyperaldosteronism, glucocorticoid-induced aldosteronism, 11-beta hydroxylase deficiency, adrenergic, 17-α Hydroxylase deficiency, secretion of adrenergic ectopic tumor, renal parenchymal disease, and the like. Elevation is seen in high essential hypertension. Renin type, malignant hypertension, Barth syndrome, vascular hypertension, pregnancy, cirrhosis edema, adrenal insufficiency, low sodium diet, juxtaglomerular cell tumor and the like. Low results may be diseases: high potassium hyperglycemia results in the elderly may be diseases: precautions for hypokalemia in the elderly (1) Determination of plasma renin activity, the diet of the subject should control sodium and potassium intake, the standard diet is daily: sodium 100mmol, potassium 60 ~ 100mmol. (2) Oral contraceptives can increase plasma renin activity, and mineral corticosteroids, licorice, etc. can be used to reduce the measurement results. Inspection process (1) Number the test tubes. (2) Specimen processing: The frozen specimen plasma is thawed in flowing cold water, and double specimens are quickly taken. One part of the control tube was placed in a 4 ° C water bath. One part of the measuring tube was kept in a 37 ° C water bath for 1 h, taken out and placed in an ice water bath. (3) Loading (this operation should be carried out in an ice water bath). (4) Shake well after the addition, first measure the total radioactivity of each tube, then centrifuge at 4000r/min for 15min, and aspirate the supernatant. (Note: Be sure to suck it up, you can take it 1 time, then take it again after a while). The radioactivity Bi precipitated in each tube was measured. Note: Mix normal human serum to be fresh. Not suitable for the crowd Generally no taboos. Adverse reactions and risks Risk of infection: If you use an unclean needle, you may be at risk of infection.