Serum Apolipoprotein Determination

The protein part of plasma lipoprotein is called apolipoprotein, which is mainly divided into five types, A, B, C, D, and E. It is mainly synthesized in the liver (partially in the small intestine), and apolipoprotein is an important component of plasma lipoprotein. ApoCII deficiency is an autosomal recessive disorder. Plasma ApoCII levels in heterozygotes are only half of normal, plasma triglyceride concentration can still maintain normal, homozygous plasma ApoCII is completely lacking, although LPL structure is normal, plasma ApoCII is still up to 10g / L, showing type I high lipoprotein Hypertension can cause hepatosplenomegaly in severe cases and induce acute pancreatitis. Patients in need of examination for patients with hyperlipoproteinemia. Basic Information Specialist classification: growth and development check classification: biochemical examination Applicable gender: whether men and women apply fasting: fasting Tips: It is forbidden to take certain drugs (such as birth control pills, thyroid hormones, steroid hormones, etc.) before the test to affect blood lipid levels. Normal value The minimum detection amount is 25 ng, the standard curve working range is 1.5 to 30.0 mg%, the intraplate and interplate variation coefficients are 6.5 to 7.8% and 6.6 to 11.0%, respectively, and the recovery rate is 107.5%; 185 normal human serum apoCII content, Male 5.1 ± 1.9 mg% (n = 95), female 4.8 ± 1.7 mg% (n = 90). Clinical significance Abnormal results: ApoCII deficiency is an autosomal recessive disorder. Plasma ApoCII levels in heterozygotes are only half of normal, plasma triglyceride concentration can still maintain normal, homozygous plasma ApoCII is completely lacking, although LPL structure is normal, plasma ApoCII is still up to 10g / L, showing type I high lipoprotein Hypertension can cause hepatosplenomegaly in severe cases and induce acute pancreatitis. People who need to be examined: patients with hyperlipoproteinemia. Low results may be diseases: high results of three high symptoms may be diseases: high blood lipids, fat malnutrition precautions Forbidden before examination: It is forbidden to take certain drugs (such as birth control pills, thyroid hormones, steroid hormones, etc.) to affect blood lipid levels. Requirements for examination: There should be no history of acute illness, injury or surgery in the near future. Inspection process Subjects were venously collected and assayed in time for serum separation. Analysis of the terminal amino group is one of the methods for identifying the purity of the protein. A protein of a peptide chain, through the quantitative analysis of the N-terminus, should have an integer number of N-terminal amino acids per gram of protein, and the presence of a small amount of other terminal amino acids often indicates the presence of impurities. Analysis of the protein's various amino acid percentages can also be used as a reference for the identification of various proteins. The final purity standard should be the determination of the amino acid sequence. Not suitable for the crowd 1. Patients who have taken contraceptives, thyroid hormones, steroid hormones, etc., may affect the results of the examination and prohibit patients who have recently taken the drug history. 2, special diseases: patients with hematopoietic function to reduce disease, such as leukemia, various anemia, myelodysplastic syndrome, etc., unless the examination is essential, try to draw less blood. Adverse reactions and risks 1, subcutaneous hemorrhage: due to pressing time less than 5 minutes or blood draw technology is not enough, etc. can cause subcutaneous bleeding. 2, discomfort: the puncture site may appear pain, swelling, tenderness, subcutaneous ecchymosis visible to the naked eye. 3, dizzy or fainting: in the blood draw, due to emotional overstress, fear, reflex caused by vagus nerve excitement, blood pressure decreased, etc. caused by insufficient blood supply to the brain caused by fainting or dizziness. 4. Risk of infection: If you use an unclean needle, you may be at risk of infection.