Determination of insulin-c-peptide and its release test

Insulin-c peptide assay and its release test are methods for measuring clinical function of pancreatic islet B cells by measuring fasting and postprandial plasma insulin and C-peptide levels. Since insulin and C-peptide share a common precursor, proinsulin, one molecule of proinsulin is decomposed into one molecule of insulin and one molecule of C-peptide under special action and released into the blood. The half-life of insulin is 4-5 minutes, while the half-life of C-peptide is about 10-13.5 minutes long, and 50% of insulin is cleared by the liver, the clearance of C-peptide is very low, and the C-peptide is not external. The effect of source insulin, therefore, for patients receiving insulin therapy should pay more attention to C-peptide levels to understand the function of islet B cells. Basic Information Specialist classification: growth and development check classification: endocrine examination Applicable gender: whether men and women apply fasting: fasting Tips: Check before taboo: pay attention to normal diet, pay attention to normal work and rest, prevent endocrine disorders. Normal value After normal people take glucose, plasma insulin and C-peptide also rise rapidly with the rise of blood sugar, which usually peaks in 30-60 minutes, and is 5-10 times of fasting level, then gradually decreases, reaching fasting level 3 hours after meal. That is, it is secreted synchronously, indicating that the islet B cells have a strong reserve function. Clinical significance Abnormal results: 1, type diabetes due to severe destruction of islet B cells, fasting plasma insulin and C-peptide levels are very low, no significant increase after sugar stimulation, showing a low flat curve, some patients may not even detect; 2, type 2 diabetes fasting plasma insulin and C-peptide levels can be normal or slightly higher, after insulin stimulation can secrete insulin and C-peptide but showed a peak delay. Obese or overweight type 2 diabetics, fasting plasma insulin and C-peptide levels higher than normal weight, postprandial secretion is also higher, even more than normal, but still lower than the same weight of non-diabetic, so called insulin Relatively insufficient. Abnormal symptoms of diabetes: (1) Dry mouth, thirst, drinking water, blemishes, ecchymosis, edema, swelling of the gums, pain in the teeth, or burning sensation in the mouth; (2) Weight and weight are slowly reduced, and there is no obvious cause; (3) physical fatigue, often hunger, sweating, fatigue, palpitations, trembling, hypoglycemia; (4) Urine: male frequent urination, more urine; (5) There are yellow flat new organisms (yellow tumors) under the eyes of the eyelids; (6) The skin lower limbs and foot ulcers are unhealed for a long time; or there are repeated skin and vulvar infections; skin abrasions or scratches are not easy to heal, or there are repeated balanitis, vulvitis, vaginitis; (7) vascular arteriosclerosis, hypertension, coronary heart disease; (8) Reproductive women have multiple abortions, pregnancy poisoning, excessive amniotic fluid, or a large fetus. The people who need to be examined are: patients with diabetes-related symptoms. Precautions Forbidden before examination: pay attention to normal diet, pay attention to normal work and rest, and prevent endocrine disorders. Requirements for inspection: Actively cooperate with the doctor's request. Inspection process Plasma insulin and C-peptide levels can be measured by fasting and 1 hour, 2 hours, and 3 hours postprandial to reflect islet B cell function and guide clinical treatment. After normal people take glucose, plasma insulin and C-peptide also rise rapidly with the rise of blood sugar, which usually peaks in 30-60 minutes, and is 5-10 times of fasting level, then gradually decreases, reaching fasting level 3 hours after meal. That is, it is secreted synchronously, indicating that the islet B cells have a strong reserve function. Performing islet function testing helps to understand the storage function of islet B cells and is the most reliable method for diagnosing diabetes and distinguishing between types of diabetes. Not suitable for the crowd There are no special taboos. Adverse reactions and risks No related complications and hazards.