Anti-Islet Cell Antibody (ICA)

The anti-islet cell antibody is an organ-specific antibody, and the antigen is an islet cell cytoplasm component or a microsomal component, mainly lgG, which is a marker of beta cell damage in islet cells. Anti-islet cell antibodies are usually determined by indirect immunofluorescence, and the fluorescent pattern is characterized by the cytoplasm of islet cells stained with spots. Basic Information Specialist classification: Digestive examination classification: endocrine examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: normal. Positive: diabetes. Tips: Do not eat too greasy, high-protein foods the day before the blood draw, avoid heavy drinking. The alcohol content in the blood directly affects the test results. Normal value Indirect immunofluorescence was normal in human serum anti-islet cell antibodies. (Note the specific reference value depends on each laboratory.) Clinical significance Abnormal results: (1) Diagnosis of insulin-dependent diabetes. In patients with insulin-dependent diabetes, the detection rate is 60% to 70%. It can often be measured in the early stage of clinical onset and decreased in a few weeks. The detection rate was about 20% in 3 years after onset. (2) As a classification indicator for diabetes. The positive rate of IDDM patients was 65%-85%, while the positive rate of NIDDM patients was about 10%. (3) Judging non-insulin dependent diabetes outcomes. The clinical manifestations of patients with NIDDM, ICA still indicates the occurrence of IDDM, secondary oral hypoglycemic agents failure indicators. (4) As a monitoring index after islet transplantation. After allogeneic pancreas transplantation, ICA in the blood of patients is prone to rejection of the graft. Note that the detection rate of ICA and GADA in patients with type 1 diabetes is positively correlated. The agreement rate of the two antibodies is 90% in the early stage of the disease and 54% in the disease process. Patients with insulin-dependent diabetes mellitus, diabetes, and NIDDM need to be examined. Positive results may be diseases: considerations for diabetes Forbidden before examination: Please inform the doctor about the recent medication and special physiological changes before the test. 1, do not eat too greasy, high-protein food the day before the blood, to avoid heavy drinking. The alcohol content in the blood directly affects the test results. 2. After 8 pm on the day before the medical examination, you should start fasting for 12 hours to avoid affecting the test results. Requirements for examination: When taking blood, you should relax your mind, avoid the contraction of blood vessels caused by fear, and increase the difficulty of blood collection. Obtain serum samples and store at -20 degrees Celsius. Inspection process Indirect immunofluorescence experimental principle: fluorescein is labeled on the corresponding antibody and directly reacts with the corresponding antigen. In the first step, an unknown unlabeled antibody (sample to be tested) is added to a known antigen sample, and incubated at 37 ° C for 30 min in a wet box to sufficiently bind the antigen antibody, followed by washing to remove unbound antibody. In the second step, a fluorescently labeled anti-globulin antibody or an anti-IgG, IgM antibody is added. If an antigen-antibody reaction occurs in the first step, the labeled anti-globulin antibody is further bound to the antigen-binding antibody, thereby enabling identification. Unknown antibody. Not suitable for the crowd Inappropriate people: generally no special population. Adverse reactions and risks No special complications or hazards.