Serum total complement activity (CH50)

Complement has the functions of lysing target cells, promoting phagocytosis, and participating in inflammatory reactions. At the same time, complement plays a key role in immune regulation, clearing immune complexes, stabilizing the body environment, participating in allergic reactions and autoimmune diseases. Changes in total complement activity and individual complement components are important for the diagnosis and efficacy of certain diseases. The determination of total complement primarily reflects the active program of complement activation via traditional pathways. Clinically, the test tube method is commonly used. Basic Information Specialist classification: cardiovascular examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Found in acute glomerulonephritis, membrane proliferative glomerulonephritis, systemic lupus erythematosus (SLE) active, rheumatoid arthritis, viral hepatitis, chronic liver disease, subacute bacterial endocarditis, hereditary vascular nerve Sexual edema, etc. Normal value: Normal value: 50-100U/L Above normal: Found in acute inflammatory infections, tissue damage, acute rheumatic fever, nodular arteritis, dermatomyositis, myocardial infarction, typhoid, polyarthritis, cancer and so on. negative: Positive: Tips: Do not eat too greasy, high-protein foods the day before the blood draw, avoid heavy drinking. The alcohol content in the blood directly affects the test results. After 8 pm on the day before the medical examination, fasting should be done to avoid affecting the detection of indicators such as blood glucose in the second sky. Normal value 50~100U/L. Clinical significance Increased in acute inflammatory infections, tissue damage, acute rheumatic fever, nodular periarteritis, dermatomyositis, myocardial infarction, typhoid, polyarthritis, cancer and so on. Reduced in acute glomerulonephritis, membrane proliferative glomerulonephritis, systemic lupus erythematosus (SLE) active, rheumatoid arthritis, viral hepatitis, chronic liver disease, subacute bacterial endocarditis, hereditary blood vessels Neuropathic edema, etc. High results may be diseases: pediatric vascular immunoblastic lymphadenopathy, hereditary complement deficiency disease, pediatric serum disease, pediatric hereditary angioedema, hepatitis B virus arthritis, picornavirus keratitis considerations First, the precautions before blood draw 1, do not eat too greasy, high-protein food the day before the blood, to avoid heavy drinking. The alcohol content in the blood directly affects the test results. 2. After 8 pm on the day before the physical examination, fasting should be done to avoid affecting the detection of indicators such as blood glucose in the second sky. 3, should relax when taking blood, to avoid the contraction of blood vessels caused by fear, increase the difficulty of blood collection. If you have a history of fainting blood, please explain in advance and make special arrangements. Second, should pay attention after blood draw 1. After blood is drawn, local compression is required at the pinhole for 3-5 minutes to stop bleeding. Note: Do not rub, so as not to cause subcutaneous hematoma. 2, the pressing time should be sufficient. There is a difference in clotting time for each person, and some people need a little longer to clotting. Therefore, when the surface of the skin appears to be bleeding, the compression is stopped immediately, and the blood may be infiltrated into the skin due to incomplete hemostasis. Therefore, the compression time is longer to completely stop bleeding. If there is a tendency to bleed, the compression time should be extended. 3, after the blood draw symptoms of dizziness, such as: dizziness, vertigo, fatigue, etc. should immediately lie down, drink a small amount of sugar water, and then undergo a physical examination after the symptoms are relieved. 4. If there is localized congestion, use a warm towel after 24 hours to promote absorption. Inspection process (1) The serum to be tested is diluted 1:20, and 0.2 ml of the serum to be tested is aspirated into a test tube, and 3.8 ml of barbital buffer is added and mixed. (2) Take 10 tubes and mix with liquid. (3) First compare the above tubes with the 50% hemolysis standard tube, select two tubes close to the standard tube, and read with a spectrophotometer at a wavelength of 542 nm to find a closer to the standard optical density. One tube. The total complement value was determined according to the standard based on the amount of diluted serum added to the tube. Not suitable for the crowd There are no special taboos. Adverse reactions and risks No related complications and hazards.