Specific lipase staining

The positive reaction appears only in granulocytes at various stages, and is more specific than POX. AS-DCE is also known as granulocyte-specific esterase. The chloroacetic acid AS-D naphthol esterase in granulocytes hydrolyzes the chloroacetic acid AS-DCE naphthol in the matrix fluid to produce naphthol AS-D, which is then coupled with a stable diazonium salt to form an insoluble red precipitate, which is localized In the cytoplasm.