Acridine orange immunofluorescence

This method is based on the specificity of serology and the sensitivity of fluorescence method, combined with separation and culture, and accelerates agglutination through the action of low-speed centrifugal precipitation and highly diluted fluorescent pigments. It can achieve the purpose of increasing the bacterial clot and reducing the cross-agglutination reaction of the generic antigen-antibody. Due to the selective agglutination of the antigen-antibody, the interference of impurities and impurities in the specimen can be eliminated, and the Live bacteria were isolated in order to make a precise diagnosis, thus increasing the specificity, sensitivity and rapidity of the method. According to the preliminary screening result of the fluorescent bacteria group, it can be completed within 30 minutes. From the isolation and cultivation of the fluorescent bacteria group to the exact diagnosis, it can be completed within 14-18 hours, which is 20-30 hours ahead of the conventional culture method. The positive rate of isolated bacteria is about 3 times higher than that of conventional methods. In addition, this method does not need to extract Ig, and it can be mixed with immune serum to make a diagnostic solution. The method is simple and easy to store for a long time. This method has been used for the inspection of porcine erysipelas, salmonella, campylobacter jejuni, and perfringens.