Protein electrophoresis

In an alkaline environment, serum proteins are negatively charged, and they swim to the anode in the electric field. Due to the differences in isoelectric point and molecular weight of each protein, small molecular weights and more negative charges are faster; large molecular weights and less negative charges People swim slower. After electrophoresis, starting from the anode, there are five zones of albumin, a1 globulin, a2 globulin, β globulin and γ globulin.