Anti-neuronal nuclear antibody

Anti-neuronal nuclear antibodies are divided into type 1 and type 2, type 1 is also called Hu antibody, and type 2 is also called Ri antibody. Hu's target antigen is present in all neurons of the central and peripheral nervous system, as well as in related tumor cells. Ri's target antigen is limited to neurons of the central nervous system. No anti-neuronal nuclear antibodies were found in normal people. Hu antibodies are more common than Ri antibodies. Clinically, indirect immunofluorescence is used to determine anti-neuronal nuclear antibodies. Principle of indirect immunofluorescence method: Label fluorescein on the corresponding antibody and directly react with the corresponding antigen. In the first step, add an unknown unlabeled antibody (sample to be tested) to a known antigen sample, and incubate at 37 ° C for 30 minutes in a wet box to fully bind the antigen and antibody, and then wash to remove the unbound antibody. In the second step, add fluorescently labeled antiglobulin antibodies or anti-IgG and IgM antibodies. If an antigen-antibody reaction occurs in the first step, the labeled antiglobulin antibody will further bind to the antigen-bound antibody, which can identify unknown antibodies.