Anti-insulin receptor antibody

Anti-Insulin Receptor Antibody (IRA) was discovered by Flier in 1975 when studying patients with insulin resistance syndrome with acanthosis nigricans. This antibody can bind to insulin receptors present on the cell membrane of the body, and shows a reduced affinity for insulin. When detecting IRA, it is common to pass human lymphocyte IM-9 (receptors on the cytoplasmic membrane are abundant and cells are easily available) or human placenta to prepare insulin receptor antigens. Commonly used methods are radioreceptor analysis (RRA) and immunoprecipitation tests.