Serum pyruvate kinase

Pyruvate kinase catalyzes the conversion of phosphoenolpyruvate and ADP to pyruvate and ATP. It is one of the main rate-limiting enzymes in glycolysis. There are two types of isoenzymes, M and L M type has M1 and M2 subtypes. M1 is distributed in cardiac muscle, skeletal muscle and brain tissue; M2 is distributed in brain and liver tissue. L-type isoenzymes are mainly found in liver, kidney and red blood cells. After myocardial cell necrosis, PK is released into the blood, and the measurement of PK can be used to diagnose myocardial infarction.