Anti-liver and kidney microsomal antibody

Anti-liver and kidney microsomal antibody assay (LKM) is commonly used for indirect immunofluorescence identification. Principle of indirect immunofluorescence method: Label fluorescein on the corresponding antibody and directly react with the corresponding antigen. Indirect immunofluorescence technology has found anti-hepatic / renal microsomal antibodies (anti-LKM) in some patients with chronic hepatitis. These antibodies can react with hepatocyte cytoplasm and proximal renal tubules. Antimitochondrial antibodies to primary biliary cirrhosis can stain distal tubules. Subsequently, three subtypes of LKM antibodies were identified. LKM-1 is a serological marker of type II autoimmune hepatitis. LKM-2 only appears in drug-induced hepatitis caused by tannic acid, so LKM-1 must be associated with LKM-2 is different. LKM-3 mainly appears in some patients with chronic hepatitis D.