Serum Mucin (SM)

Mucin is a complex protein that binds to mucopolysaccharides and proteins. It belongs to a kind of glycoprotein in the body. It migrates together with α-globulin during electrophoresis, mainly in α1 and α2 globulin. Determination of serum mucin has a certain reference value for the diagnosis of liver and kidney diseases and the dynamic observation of some diseases and the course of disease. Basic Information Specialist classification: cardiovascular examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Tips: It is advisable to take fasting venous blood and test fresh samples. Normal value The protein is 710 to 870 mg/L (71 to 87 mg/dl). 33.8 ± 2.7 mg / L (3.38 ± 0.27 mg / dl) in terms of tyrosine. Clinical significance 1, elevated in acute and chronic inflammation (such as tuberculosis, pneumonia, pleurisy, subacute bacterial endocarditis, etc.), malignant tumors (especially female reproductive tract tumors), connective tissue diseases (such as rheumatic fever, rheumatoid joints) Inflammation, gout, etc.), obstructive jaundice, diabetes, liver cancer, etc., can also be found in burns and pregnancy. 2, reduced in liver parenchymal lesions (viral hepatitis, toxic hepatitis, hepatic jaundice, cirrhosis), nephrotic syndrome, pituitary and chronic adrenal insufficiency, hyperthyroidism or hypothyroidism, visceral degeneration, vascular degeneration, etc. . Low results may be diseases: pleurisy, cirrhosis, malignant tumors may result in high disease Possible diseases: Behcet's syndrome, stasis subcutaneous sclerosis considerations 1. Fasting venous blood should be taken and fresh samples should be tested. 2. Taking anabolic steroids, L-asparaginase, etc. can make the measurement results high; oral contraceptives can reduce the results. 3. Continuous monitoring of mucin in the same case can be used for dynamic observation of the course of disease, and has certain reference value for the course of disease, such as the severity of the disease, the size of the tumor, whether it is metastasized, surgical resection or other treatment effects. Inspection process Serum 0.5ml, add 154mmol / L sodium chloride 4.5ml, mix, add 2.5ml / L perchloric acid solution 2.5ml, stand still for 10min, filter or centrifuge with quantitative filter paper. 2.5 ml of the filtrate was taken, and 0.5 ml of 17.74 mmol/L phosphotungstic acid was added thereto, and the mixture was allowed to stand for 10 minutes, and centrifuged at 3000 rpm for 10 minutes. The supernatant was decanted and drained, and 2 ml of a phosphotungstic acid solution was added to suspend the precipitate. After centrifugation, the supernatant was decanted and drained. Take the precipitate for later use. Determine according to Table 1. Mix well, place a 37 ° C water bath for 15 min, take out a spectrophotometer 650 nm, cuvette light path 1.0 cm, blank zero, read the absorbance of each tube. Not suitable for the crowd Special diseases: Patients with hematopoietic dysfunction, such as leukemia, various anemia, myelodysplastic syndrome, etc., unless the examination is essential, try to draw less blood. Adverse reactions and risks 1, local subcutaneous hemorrhage: after the blood collection should be pressed for a sufficient time, especially those with bleeding tendency, so as not to cause subcutaneous oozing and bruising due to no blood coagulation. 2, infection: attention to aseptic operation during venous blood collection, so as not to cause local infection.