Total bone marrow nucleated cells

Under normal circumstances, the whole process of development and evolution of blood cells from primitive to mature stage has certain regularity. 1 The size of the cell body matures with the development of blood cells, and the cell body gradually becomes larger and smaller (the opposite of megakaryocytes). The more mature the cell, the larger the cell body. 2 The nucleus is large to small, the mature red blood cells are non-nuclear, the nucleus is round to irregular, the granules are finally lobulated, the lymphocytes and plasma cell lines are not changed, the chromatin is from fine to loose and coarse, and the nuclear membrane From not obvious to obvious, nucleoli from nothing to nothing. The quality of 3 cells is from small to large, and the lymphocyte changes are not obvious. Wright's staining and Giemsa staining show that the cytoplasm is dark blue to light blue, and mature granulocytes and red blood cells can be turned into pink and red, and the particles are free. 4 The ratio of nuclear to cytoplasmic volume is from large to small. In the pathological situation, the above evolutionary law can be disordered. Basic Information Specialist classification: growth and development examination classification: blood examination Applicable gender: whether men and women apply fasting: not fasting Tips: Preoperative preparation: The patient is instructed to position the patient according to the doctor's instructions. Normal value (10 to 180) × 109 / L (1 to 180,000 / mm3). Clinical significance Abnormal results: (1) Increased bone marrow hyperplasia, such as leukemia, hemolytic anemia, hypersplenism and so on. (2) Reduce the dysfunction of hematopoietic tissue, such as aplastic anemia. Children or adolescents with sudden onset of fever, progressive anemia, significant bleeding tendency or bone and joint pain in the early stage of the disease; slow onset of progressive fatigue, pale, tired and shortness of appetite, lack of appetite, Elderly and some young patients with symptoms of weight loss or fever of unknown origin; people with symptoms of anemia. Low results may be diseases: high aplastic anemia results may be diseases: hemolytic anemia, leukemia considerations People who are not suitable for examination: hemophilia and disseminated intravascular coagulation. If there is no special need, do not have a bone marrow puncture. Preoperative preparation: The patient is placed in accordance with the doctor's instructions. Inspection process Inspection method: bone marrow examination. Inspection process: 1. Select the puncture site. 2. Anesthesia. 3. Fix the length of the needle. 4. The doctor's left thumb and finger are fixed at the puncture site. The right hand-held bone marrow puncture needle is inserted perpendicularly to the bone surface. If the sternum is puncture, it should be inserted at an angle of 30o to 40o with the bone surface. When the needle tip touches the bone, rotate the needle along the long axis of the needle of the needle and push it forward to slowly penetrate the bone. 5. Extract the bone marrow fluid and pull out the needle core, connect the dry syringe (10m1 or 201m1), and use the appropriate force to extract the bone marrow fluid. 6. Smear: It is required that the smear slide and push piece should be clean, no putty pollution, the smear should be thin and uniform, the number of smears is about 10, and two blood samples are used for comparison. 7. Staining: Commonly used Wright-Gemsa mixed staining method, cytochemical staining is often used together. 8. Microscopic observation.