Syphilis Qualitative Test

When the body is infected with Treponema pallidum, it produces Treponema pallidum antibodies, including specific antibodies and non-specific antibodies (reactants). The responsiveness test is a qualitative test, and the specific antibody test (treponema pallidum hemagglutination test) contributes to the diagnosis of syphilis. Precautions for syphilis qualitative test: inappropriate population: generally detected by syphilis infection patients. Contraindications before examination: The specimen should be kept clean. Blood samples should not be used for hemolyzed or contaminated blood specimens. Requirements for inspection: Generally, after a high-risk sexual life, it is generally required to do regular inspection as soon as possible. Basic Information Specialist classification: Infectious disease examination and classification: immunological examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: normal. Positive: Infected with syphilis. Tips: Generally, after a high-risk sex life, it is generally required to do regular inspection as soon as possible. Normal value A positive reaction (+++~++++) showed a medium or large red agglomerate. 2 weak positive reaction (+ ~ ++) visible smaller red agglomerates. 3 Negative reaction (-) visible uniform antigen particles without agglomerates. The normal value is negative. Clinical significance Abnormal results: 1 qualitative test for the primary screening of syphilis, because the antigen of the above test is non-specific, so some non-syphilitic diseases such as systemic lupus erythematosus, rheumatoid arthritis, scleroderma, leprosy, etc. can be false positive. Pregnant women can also be positive. 2 Under the premise of positive qualitative test, the specific antibody test positive can be diagnosed as syphilis. People who need to be examined: Patients with syphilis infection need to be examined. Or suspect that the patient can also be examined. Positive results may be diseases: congenital syphilitic cirrhosis, pregnancy with syphilis, pediatric yaw disease considerations Contraindications before examination: The specimen should be kept clean. Blood samples should not be used for hemolyzed or contaminated blood specimens. Requirements for inspection: Generally, after a high-risk sexual life, it is generally required to do regular inspection as soon as possible. Inspection process (1) Qualitative test: 1 A 50 ul syphilis positive control and a negative control were separately applied to the two circles of the paper card. 2 Take 50 ul of serum or plasma to be tested (without inactivation) in another circle of the paper card. 3 Drop the TRUST reagent 1 drop vertically into the above serum with a special dropper and a needle. 4 Shake for 8 minutes at 100 rpm, and observe the titer with the naked eye. (2) Quantitative test: The serum to be tested was diluted with physiological saline, and then tested according to the above qualitative method to exhibit the highest dilution of the apparent agglutination reaction as the agglutination titer of the serum. Not suitable for the crowd There are no special taboos. Adverse reactions and risks There are no related complications and hazards.