Hepatitis B virus e antibody (HBeAb)

Hepatitis B e antibody was positive for several months after the hepatitis B e antigen was negative. In general, hepatitis B e antibody positive indicates that the patient's infectivity has been significantly or relatively reduced. The degree of viral replication has decreased or has been significantly alleviated. In recent years, many hepatitis B e antibodies have been found to be positive, but hepatitis B virus deoxyribonucleic acid (HBV DNA) is also a positive heterotype of hepatitis B patients, and their condition has not healed. This is caused by a hepatitis B virus that is infected with mutations and cannot be ignored clinically. However, some scholars believe that the production of hepatitis B antibody only means that most of the hepatitis B virus is cleared, but it does not mean that chronic hepatitis is cured. Hepatitis B e antibody is not a protective antibody, and the appearance of e antibody generally indicates a decrease in hepatitis B virus replication or has basically stopped. If hepatitis B patients are e-positive and can not be taken lightly, it is necessary to make further judgment based on hepatitis B HBV-DNA test. Basic Information Specialist classification: Infectious disease examination and classification: blood examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: normal. Positive: Hepatitis B virus infection has entered the late stage, when the virus replication is reduced and the infection is weakened. The recovery period after chronic hepatitis B virus infection or chronic infection can be positive. Tips: If the examiner wants to check liver function (hepatitis B ultrasound) at the same time, it must be fasted. Normal value negative. Clinical significance It is used to determine the activity of infectious or viral replication of hepatitis B (hepatitis B). 1. HBeAb positive indicates a decrease in the level of hepatitis B virus replication and a decrease in infectivity. 2. "Little Sanyang" HBeAb (+), HBsAg (+), HBcAb (+), prompt (1) In most cases, it indicates a reduction in hepatitis B virus replication. But still contagious. (2) The shift from “big three yang” to “small three yang” does not mean that hepatitis B virus replication completely stops. A small number of “small three yang” patients have positive serum HBV-DNA, active virus replication, serious disease, and rapid progress. Seen in the virus variation. Positive results may be diseases: chronic hepatitis, liver fibrosis, cirrhosis The results of the examination must be combined with other hepatitis B inspection items for a comprehensive analysis. Inspection process (l) Experimental preparation: The kit was taken out from the refrigerated environment, equilibrated at room temperature for 30 min, and the concentrated washing solution was diluted 1:20. (2) Add the sample to be tested to 0.05 ml per well of the specimen to be tested, and set 2 wells for the positive control and 1 well for the blank control for the negative control. (3) Add 0.05 ml per well to the neutralizing reagent, and the blank control well is not added. (4) Add 0.05 ml of the enzyme conjugate per well, blank control wells are not added, mix well, incubate at 37 ° C for 30 min. (5) Washing machine wash plate Select washing process 5 times to wash the plate, and finally pat dry. (6) Add coloring agent first to add coloring agent A, 0.05ml per well; add coloring agent B, every 0.05ml; mix thoroughly, place at 37 °C and incubate for 15 minutes in the dark. (7) Stop the reaction Add 0.05 ml of stop solution to each well and mix. (8) Determination of the reading with the microplate reader, you can choose a single wavelength of 450nm (with blank holes to zero) Or dual wavelength 450/630, read the OD value of each well. Not suitable for the crowd People with reduced hematopoietic function such as leukemia, various anemia, myelodysplastic syndrome, or people with thrombocytopenia should pay attention to blood draw, and should not take more or more blood. Adverse reactions and risks 1. After the blood is drawn, do not press the needle hole to avoid subcutaneous hematoma. If there is a small piece of bruise in the blood, it is slightly tender, please don't panic, you can do hot compress after 24 hours to promote the absorption of blood. The general small amount of congestion will gradually absorb in 3 to 5 days and the color will become lighter and return to normal. 2. After the blood draw, symptoms such as dizziness, vertigo, fatigue, etc. should be immediately supine, drink a small amount of syrup, and then undergo a physical examination after the symptoms are relieved.