Reverse transcription-polymerase chain reaction

The principle of Reverse Transcription-Polymerase Chain Reaction (RT-PCR) is: to extract total RNA from tissues or cells, use the mRNA as a template, and use Oligo (dT) or random primers to use reverse transcription The enzyme is reverse transcribed into cDNA. Then use cDNA as a template for PCR amplification to obtain the target gene or detect gene expression.