Anti-epidermal basement membrane antibody

Anti-epidermal basement membrane antibody assay is a specific marker antibody for diagnosis of pemphigoid. With indirect immunofluorescence examination, anti-epidermal basement membrane antibodies, mainly IgG, were diagnosed in the serum of patients with pemphigoid. Principle of indirect immunofluorescence method: Label fluorescein on the corresponding antibody and directly react with the corresponding antigen. In the first step, add an unknown unlabeled antibody (sample to be tested) to a known antigen sample, and incubate at 37 ° C for 30 minutes in a wet box to fully bind the antigen and antibody, and then wash to remove the unbound antibody. In the second step, add fluorescently labeled antiglobulin antibodies or anti-IgG and IgM antibodies. If an antigen-antibody reaction occurs in the first step, the labeled antiglobulin antibody will further bind to the antigen-bound antibody, which can identify unknown antibodies.