Aspartase aminotransferase isoenzyme

AST has two isoenzymes, namely s-AST in liver cytoplasm and m-AsT in liver mitochondria. s-AST is easily released into the blood, m-AST is tightly bound to the cell substructure and difficult to release into the blood, so s-AST accounts for 60% to 80% in serum. In mild and moderate hepatocyte damage, only soluble enzymes are released from the cytoplasm of the liver; if hepatocyte damage is severe or even necrotic, enzymes in the mitochondria are also released. M-AST was used as an indicator of severe liver damage and hepatocyte necrosis.