Two-way agar diffusion test

The two-way agar diffusion test is often used for qualitative detection and semi-quantitative detection. Add the antigen and antibody to the adjacent wells on the agar gel plate, and let them spread to each other. When the two meet at the most appropriate ratio, a clear precipitation line is formed. Depending on the presence or absence of a precipitation line, unknown antibodies can be identified with known antibodies, or unknown antibodies can be identified with known antigens. This method is commonly used in clinical practice to check the alpha-fetoprotein in the serum of patients with primary liver cancer as an early auxiliary diagnosis of primary liver cancer.