Anti-keratin antibody

Anti-keratin antibody (AKA), Young et al. Found in 1979 that rheumatoid arthritis (RA) serum has an antibody that can react with the rat esophagus stratum corneum, and has specificity for rheumatoid arthritis (RA), named For AKA. In 1989, Vincent et al proposed that it should be more appropriate to rename AKA as an anti-cuticle antibody. AKA can appear several years before the onset of RA, so it has early diagnostic value. AKA detection method: Take the middle and lower 1/3 segments of 6-week-old male Wistar rats as antigens, make frozen sections, thickness 4μm ～ 5μm, and store at -70 ℃ for future use. Add 1:20 diluted serum, incubate at 37 ° C for 30 minutes in a wet box, rinse with PBS and blow dry, add 1:20 diluted fluorescein-labeled goat anti-human IgG, incubate at 37 ° C for 30 minutes, rinse, blow dry, and buffer glycerol The plate was observed under a fluorescent microscope. The criterion is that the stratum corneum has a typical regular linear or laminar fluorescence.