Bacteriological examination of pus and wound infection specimens

First, pus and wound secretions Bacterial culture of pus and wound secretions is the most common in the process of infection. The culture of pus and wound secretion bacteria is used for the diagnosis of pathogenic bacteria. The source of pathogenic bacteria in pus and wound secretion bacteria are exogenous and endogenous. There are many types of bacteria that can be detected from pus and wound secretions. The most preferred pathogenic bacteria are Staphylococcus aureus and Streptococcus pyogenes, followed by Pseudomonas and Enterobacter bacteria. Specimens were collected using direct smear, normal bacterial culture, and anaerobic culture procedures for testing. 2. Collection of Specimens 1. First, clean the contaminated bacteria on the surface of the lesion with sterile saline. 2. For ulcerated abscesses, sterile cotton swabs are usually used to take pus and secretions from the deep part of the lesion, while fistulas are taken aseptically from tissue fragments and placed in sterile test tubes for inspection. 3. For undisrupted abscesses, it is best to use 2.5% to 3% iodine and 75% alcohol to disinfect the skin of the affected part, and then extract the pus and secretions with a sterile syringe. You can also use a sterile cotton swab when cutting the pus take. 4. Sometimes the innermost dressing stained with pus can also be put in a sterile dish for inspection. 5. For actinomycete specimens, a sterile cotton swab is often used to squeeze the fistula, and the "sulfur-like particles" in the pus flowing out of the pus are stored in a test tube for inspection, or the sterile gauze can be inserted into the fistula. Take it out for inspection the next day.