Platelet activating factor

PAF content in the body is very low, its active concentration is 10-7 ～ 10-9mol / L, its half-life is 30s, it is not easy to capture; its structure is close to phospholipids, it is not easy to separate and purify; it also has multiple molecular genera, and its biological activity huge difference. In recent years, domestic and foreign scholars have done a lot of work. In summary, there are three major methods for PAF detection: ① biological detection methods; ② physical and chemical methods; ③ immunological methods. This section focuses on biological assays.