natural killer cell activity

Natural killer cells, also known as NK cells, are mainly characterized by non-specific killing of tumor cells and virus-infected cells. This killing effect does not depend on antibodies and complement. In vitro detection of NK cell activity is the diagnosis of NK cell function and An important means of dealing with certain diseases. Basic Information Specialist classification: cardiovascular examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: The body's immunity is declining. Normal value: Natural kill rate: 35-85% Above normal: no information yet. negative: Positive: Tips: Actively cooperate with doctors. Normal value The natural release rate requires <10%; the natural kill rate is about 35% to 85%, and the average is 60%. Clinical significance K cells are mainly distributed in peritoneal exudates, peripheral blood and spleen; NK cells are mainly distributed in bone marrow, peripheral blood and spleen. It is mainly used in patients with tumors, viral infections, parasitic infections, autoimmunity and transplant immunity. It detects the killing function of K and NK cells and plays an important role in maintaining and regulating specific immunity. Low results may be diseases: pediatric X-linked severe combined immunodeficiency disease, acquired immunodeficiency syndrome, amoebic bowel disease precautions (1) The requirement/target cell ratio is 100:1, and if the ratio is >100:1, the natural kill rate does not increase logarithmically. And the amount of specimen is also large. (2) The target cell placement time should not be too long. Due to the increase of dead cells over time, the natural release rate is not accurate. (3) The labeled 125IUdR concentration should be appropriate, and the radioactive concentration of 125IUdR is required to be 1.11×107Bq/ml. If the concentration is too low, the labeling rate is <0.1 cpm, and the detection result is affected. Inspection process (1) Effect of target cells: The separated lymphocyte suspension (effector cells) and labeled K562 cells (target cells) were added to the flat plastic test tube in a ratio of 100:1, and operated according to Table 1, and natural release was also provided. Tube (as a control tube), each specimen was made into 3 duplicate tubes. Finally, each tube was increased to 1 ml with a culture solution, centrifuged at 1500 r/min to promote the binding of the target cells, and incubate at 37 ° C in a 5% CO 2 incubator. 18h. (2) Enzymatic reaction: remove the incubation tube, discard the supernatant of each tube, add the remaining cells (2.2g / L) trypsin and DNase each 0.1m1, mix and 37 ° C water bath for 30min, promote The target cells that were attacked released 125 IUdR, and then each tube was immediately added with 0.8 ml of Hank solution to terminate the enzymatic reaction. After mixing, centrifuge at 1500 r/min for 2 min. (3) Measurement of radiation intensity and calculation: The cpm values ​​of each tube (supernatant and cell tube) were measured by a gamma counter, and calculated according to the formula. Not suitable for the crowd People with reduced hematopoietic function such as leukemia, various anemia, myelodysplastic syndrome, or people with thrombocytopenia should pay attention to blood draw, and should not take more or more blood. Adverse reactions and risks 1. After the blood is drawn, do not press the needle hole to avoid subcutaneous hematoma. If there is a small piece of bruise in the blood, it is slightly tender, please don't panic, you can do hot compress after 24 hours to promote the absorption of blood. The general small amount of congestion will gradually absorb in 3 to 5 days and the color will become lighter and return to normal. 2. After the blood draw, symptoms such as dizziness, vertigo, fatigue, etc. should be immediately supine, drink a small amount of syrup, and then undergo a physical examination after the symptoms are relieved.