Antidesmosome autoantibodies
Anti-desmosome autoantibodies are specific marker antibodies for pemphigus and are often combined with clinical, histological, and immunopathological diagnosis. There are three kinds of target antigens: Dsg1, which is the main target antigen for phyllodes pemphigoid antibodies; Dsg2, which is present in various epithelial cells; Dsg3, which is the main target antigen for pemphigus vulgaris (PV) antibodies. Anti-desmosome autoantibodies are commonly detected by indirect immunofluorescence: the staining pattern is characteristic reticulum. Principle of indirect immunofluorescence method: Label fluorescein on the corresponding antibody and directly react with the corresponding antigen. In the first step, add an unknown unlabeled antibody (sample to be tested) to a known antigen sample, and incubate in a wet box at 37 ° C for 30 minutes to fully bind the antigen and antibody, and then wash to remove the unbound antibody. In the second step, add fluorescently labeled antiglobulin antibodies or anti-IgG and IgM antibodies. If an antigen-antibody reaction occurs in the first step, the labeled antiglobulin antibody will further bind to the antigen-bound antibody, which can identify unknown antibodies.