Nucleic acid molecule hybridization

Molecular hybridization of nucleic acids is a powerful tool for the qualitative or quantitative detection of specific RNA or DNA sequence fragments. It was developed using the principle of base complementarity of nucleic acid molecules. Under conditions such as heating in a basic environment or adding a denaturing agent, the hydrogen bonds between the double-stranded DNA are broken (denatured), and the double-strands are disintegrated into two single-strands. At this time, add heterologous DNA or RNA (single-stranded) and incubate (renaturation) under a certain ionic strength and temperature. If some regions between the heterologous DNA or RNA have complementary base sequences, then renaturation When hybridized nucleic acid molecules can be formed.