Hemoglobin F inclusion body generation test
The red-red dye tar blue was added to the blood. After incubation at 37 ° C, HbH precipitated due to oxidative degeneration, was granular, stained dark blue, and was evenly and diffusely dispersed in red blood cells. In patients with HbH disease, red blood cells containing inclusion bodies can reach more than 50%. In mild thalassemia, HBH inclusions are occasionally seen.
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